Laboratory in Cell Structure-Biological Electron Microscopy

A. The students will learn basic concepts of transmission electron microscope, principles of biological sample preparation for transmission electron microscopy, various electron microscopy applications, to be familiar with the morphology of different organelles inside the mammalian cell, and understand the functions of each organelle.B. Lab projects involve: (1) perfusion fixation of mouse tissues; (2) routine transmission electron microscopy sample preparation; (3) techniques and tools used for microtomy; (4) transmission electron microscope alignment; and (5) imaging various cellular ultrastructures using a transmission electron microscope.